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Vitamin D Test Price, Procedure, Ranges & Sample Report 2025

Vitamin D Profile by Thyrocare includes test on 3 Tests parameters. It comprises of essential tests on Vitamin for an early detection of any potential illness in your body. This package which comes under VITAMIN category , now available at a discounted price of Rs. 1910

A Vitamin D test measures the level of vitamin D & diagnose Vitamin D Deficiencies in blood

Vitamin D Profile Sample Report

Vitamin D Profile : Info - Procedure

Test Price
Rs. 1910 (Tax included)
Group Name
VITAMIN
Fasting
Fasting not required
Sample Type
SERUM
Sample Collection
Expert collect samples at Home
Reports
Delivered Online within 24-48 Hrs*
Report type
Email & Hardcopy ( On request )

List of test included in Vitamin D Profile


  • Vitamin d total
  • Vitamin d2
  • Vitamin d3
Vitamin D Profile Sample Report
Full View

Vitamin D Profile Sample Report & Ranges

Conducted at Thyrocare Labs
Lab Accreditation CAP, NABL, ICMR, NGSP, ISO
Method Liquid Chromatography-Tandem Mass Spectrometry
Technology LC-MS/MS
Supervision Dr Sachin Patil MD (Path)
Normal Vitamin D Levels 20-100 ng/mL
Low Vitamin D Levels Less Than 20 ng/mL
High Vitamin D Levels Greater Than 100 ng/mL
Results Highly Specific

The optimal range of vitamin D in an average adult must be between 20 to 100 ng/ml. Levels below 20 ng/ml are low and may cause bone problems. Whereas, levels above 100 ng/ml.

 

A Vitamin D profile test contains three main parameters, including Vitamin D total, Vitamin D2, and D3. Vitamin D is essential to maintain various functions of the body. It can be maintained with a balanced diet and required supplements. 

Vitamin D blood test measures the range of the abovementioned 3 vitamins in the body. It helps you know and understand your overall health and take appropriate measures on time. This test helps in monitoring the deficiency and high levels of vitamin D in the body. If you are experiencing any deficiency symptoms such as fatigue, weakness, bone and muscle weakness, loss of appetite, and cramps, it is time for you to get a Vitamin D test near you .

What is Vitamin D Profile Test - (D2, D3 & D Total)?

A vitamin D Profile test is done to measure the levels of vitamin D in the body to ensure proper functioning and health. The test has 3 parameters, which are vitamin D2, D3 & D total. The test is based on these three vitamin levels. It helps the doctor to give an appropriate course of treatment in case there is a deficiency or toxicity due to high levels of the vitamin. Hence a total vitamin D test is advised for early detection of abnormal range and taking preventive measures.

Vitamin D profile Test

Use of Vitamin D Test

A Vitamin D Profile test is done for the following reasons:-

  • To detect a possible disease in the body.

  • For monitoring the insufficient or deficient levels of Vitamin D.

  • To diagnose patients with persistent illnesses related to insufficient vitamin D levels.

  • Regular checkups of individuals who are at risk due to anomaly high or low range of vitamin D. Risk factors might include limited or no sun exposure, bone deformity, digestive disorder, low immunity, obesity, and liver or kidney diseases.

 

Vitamin D deficiency affects many body parts and interferes with normal day-to-day functioning. Hence, if you have experienced any chronic illnesses for a long time, it is advised that you must consult a healthcare provider for a test.

 

Need for Vitamin D Test

  • The test is needed because the body needs to have sufficient levels of vitamin D to maintain good health. 

  • A deficiency of the same for a longer period may cause several diseases among children and adults.

  • With the help of the test, disorders and diseases could be detected and treated in time.

  • Based on the vitamin D test report, your doctor may be able to give a better course of treatment. 

  • Your doctor may also suggest the test in case you have diseases such as Crohn’s, celiac, or ulcerative colitis. These conditions make it difficult for the body to absorb essential nutrients.

  • Children living in areas with little sunlight, who have low levels of vitamin D, may be advised to take the test.

  • Babies require vitamin D shortly after birth. If they are being fed breastmilk, they might have a low level of vitamin D. In this case, the doctor may advise them to get a screening test done.

 

Benefits of Vitamin D Test

  • Vitamin D test will help to detect the amount of calcium in the body.

  • The test results help an individual or their healthcare provider to better guide them for further action. 

  • The advice and prescription of the doctor may help an individual to take the necessary steps to improve the levels of vitamin D. Improved levels will in turn help them to maintain their bone health, diabetes, muscles and teeth.

  • A regular checkup or assessment will help a person to maintain their health and avoid diseases.

 

Symptoms of Vitamin D deficiency & toxicity:

vitamin d normal ranges

You need to consult a doctor or get a test if you show the following symptoms due to vitamin D deficiency and too much of the vitamin:-

  • Pain and weakness in bones & muscles

  • Bone deformity

  • Mouth ulcers

  • Chronic fatigue and weakness

  • Osteoporosis(low bone density)

  • Muscle aches

  • Loss of appetite

  • Dental abnormalities

  • Muscle Cramps

  • Pale skin

  • Sleep disturbance

  • Dull skin

Causes of Vitamin D deficiency & toxicity

The deficiency of vitamin D may be caused due to various reasons, but it also depends on individual to individual. The most common causes include:

  • Not enough exposure to sunlight.

  • Insufficient intake of vitamin D in regular diet.

  • Having malabsorption disorder due to which vitamin D doesn't get absorbed in your body through diet or supplements.

  • Liver or kidney illness.

 

Normal range of Vitamin D

The optimal range of vitamin D in an average adult must be between 20 to 100 ng/ml. Levels below 20 ng/ml are low and may cause bone problems. Whereas, levels above 100 ng/ml.

 

Booking Procedure of Vitamin D Test

  • You need to search Vitamin D test near me on the internet, and it will guide you to Mediyaar’s website. 

  • Once you open the website link, you can search for the vitamin D test at the top to get complete details of the test. 

  • The same page will also provide you with a form to fill out. 

  • You have to fill in the correct details in the form. It includes name, mobile number, gender, age, email, address, pin code, appointment date, time slot, and number of persons taking the test.

  • Then you can book and proceed towards payment.

  • Once your test is booked, you will receive a payment link. You can make the payment using that. Or else, you can make the online payment when the professional arrives at your home for blood sample collection. 

 

Test Procedure of Vitamin D

The test procedure is very basic. It is a simple blood test that is done at the convenience of your home. 

  • Once your test is booked, a phlebotomist is assigned to you.

  • He will take a blood sample from your arm through the process of venipuncture.

  • The professional will make sure to clean the area on your arm with an antiseptic before this process to ensure your safety.  

  • A needle is inserted in your vein to draw a small amount of blood. You might feel a sting during the insertion. 

  • The whole process hardly takes less than 5 minutes to complete.

 

Precautions Before Vitamin D Test

There is no requirement to take special precautions before the test. However, your doctor or a healthcare professional from our team might advise you to take specific measures. It depends on individual to individual’s symptoms and medical history. So it is best to take advice before the test. 

 

How many tests are included in the Vitamin D Profile?

The vitamin D Profile Test has 3 tests:

1. Vitamin D Total Test -  Vitamin D total assesses the levels of various forms of vitamin D in the body. This test helps detect the accurate amounts of Vitamin D2 and D3. This test is recommended to detect phosphorus and calcium levels. It is also helpful to check the levels of vitamin D in individuals who have chronic illnesses such as autoimmune diseases, psoriasis, or asthma. If not checked and balanced in time, insufficient levels of vitamin D may make an individual prone to diseases such as colon cancer, breast cancer, tuberculosis, influenza, sepsis, and other infectious diseases. 

2. Vitamin D3 Test - Vitamin D3 test measures the levels of D3 in the blood. Vitamin D3 is also known as cholecalciferol. The source of Vitamin D3 is mainly animal-based foods such as milk and eggs. It can be included in the diet through supplements too. Direct sunlight is the best source to get sufficient vitamin D3. But, too much sun exposure may also cause skin damage and skin cancer. 

3. Vitamin D2 Test - Levles of D2 in the blood is measured by this test. It is a form of Vitamin D which is also known as ergocalciferol. It is a fat-soluble vitamin. The main source of vitamin D2 is plant-based or fortified foods (breakfast cereals, soy milk, bread, etc.). The Vitamin D2 test is done to detect if the body has insufficiency or abnormally high amounts of D2 to prevent diseases by doing the correct diagnosis and treatment. 

 

Eligibility for the Vitamin D Test

There are various criteria for eligibility for this test, which include:-

  • Individuals who experience symptoms of deficiency such as muscle and bone weakness, chronic fatigue, recurring infections, pain sensitivity, muscle spasms, and tingling sensation in hands and feet. It can cause rickets in children, which is a rare disease that makes the bones soft and causes them to bend.

  • People who have a deficiency of vitamin D through lack of sun exposure. Or individuals do not take any supplementation to recover that deficiency.

  • Individuals with certain medical conditions such as kidney or liver disease are also eligible for this test. Such diseases may affect the absorption of vitamin D in the body.

  • People on certain medications may also be eligible as some medications affect the absorption of Vitamin D in the body. 

Vitamin D Profile visual guide

Vitamin D Profile Reference Guide

  • Vitamin D Profile reports Vitamin D2, Vitamin D3 and their combined total in serum.
  • The total 25-hydroxyvitamin D result is the main marker used to assess Vitamin D status.
  • Separate D2 and D3 results can clarify dietary, sunlight and supplement contributions.
  • The profile does not include active 1,25-dihydroxyvitamin D or related mineral tests.
  • Interpretation should reflect symptoms, treatment, calcium balance, health conditions and the report method.

Why This Test Is Ordered

Understand the three-result profile
  • Vitamin D Profile separates 25-hydroxyvitamin D2 and D3 in serum.
  • It also reports a total representing the combined D2 and D3 contribution.
  • The total result is usually the main value for assessing Vitamin D stores.
  • Separate fractions provide extra information about the likely form entering the body.
  • D2 and D3 follow similar metabolic pathways after absorption or skin production.
  • Both forms can contribute to the circulating total 25-hydroxyvitamin D concentration.
  • Three reported values therefore describe one connected Vitamin D status pattern.
  • They should not be interpreted as three independent deficiencies or diseases.
Use total Vitamin D as the status marker
  • The liver converts Vitamin D into 25-hydroxyvitamin D for circulation and storage assessment.
  • Most routine status testing measures this 25-hydroxy form rather than active Vitamin D.
  • The total combines measured D2 and D3 contributions into the principal report value.
  • A low total can support deficiency assessment when the clinical context is appropriate.
  • One result cannot identify the reason for a low concentration.
  • Sun exposure, diet, absorption, body composition and organ health can all matter.
  • Laboratory methods and clinical guidelines may apply different interpretive thresholds.
  • Use the report range and clinician-selected goal instead of a universal target.
Read the Vitamin D2 contribution
  • Vitamin D2 is also called ergocalciferol and is available in selected supplements.
  • Some fortified foods and fungal sources can also contribute Vitamin D2.
  • The liver converts absorbed D2 into circulating 25-hydroxyvitamin D2.
  • A detectable D2 fraction may reflect diet or an ergocalciferol treatment product.
  • A low D2 fraction alone does not establish overall Vitamin D deficiency.
  • Total Vitamin D may remain adequate when the D3 contribution is sufficient.
  • D2 measurement can help evaluate response when prescribed treatment specifically contains ergocalciferol.
  • Supplement labels and prescription records are essential for meaningful D2 interpretation.
Read the Vitamin D3 contribution
  • Vitamin D3 is also called cholecalciferol and can be produced in exposed skin.
  • Fatty fish, egg yolks, fortified foods and supplements can also provide D3.
  • The liver converts D3 into circulating 25-hydroxyvitamin D3.
  • D3 often forms most of the total in people not taking Vitamin D2.
  • A measured D3 result does not quantify recent sunlight exposure precisely.
  • Season, clothing, skin pigmentation, latitude and outdoor habits affect skin production.
  • D3 supplements may raise total 25-hydroxyvitamin D differently from equivalent D2 doses.
  • Interpret the fraction beside the supplement form, dose and treatment duration.
Connect Vitamin D with bone and muscle health
  • Vitamin D supports calcium absorption and normal bone mineralisation.
  • Severe deficiency can contribute to rickets in children or osteomalacia in adults.
  • Low status may accompany bone pain, muscle weakness or fracture risk.
  • These symptoms are common and can arise from many other conditions.
  • Vitamin D testing does not directly measure bone density or fracture strength.
  • Calcium, phosphate, alkaline phosphatase and parathyroid hormone may provide related context.
  • Bone imaging or density testing answers questions not covered by this profile.
  • Treatment decisions should combine laboratory findings with age, history and clinical risk.

Symptoms And Who Should Test

People with a clinical testing indication
  • Routine Vitamin D screening is not recommended for every healthy adult.
  • Testing is more useful when it addresses a defined symptom, condition or treatment question.
  • A clinician may investigate suspected rickets, osteomalacia or abnormal calcium balance.
  • Low bone density, fragility fractures or persistent bone pain may also prompt testing.
  • Muscle weakness can support assessment but is not specific for Vitamin D deficiency.
  • Testing may be considered before or during selected bone-health treatments.
  • The reason for testing should guide which related investigations are added.
  • A screening result should not replace a complete clinical assessment.
People at risk of poor absorption
  • Conditions affecting fat absorption can reduce uptake of fat-soluble Vitamin D.
  • Coeliac disease, inflammatory bowel disease and selected pancreatic disorders may increase risk.
  • Bariatric surgery can also change Vitamin D absorption and supplement requirements.
  • Liver disease may affect conversion into circulating 25-hydroxyvitamin D.
  • Kidney disease affects later activation and overall mineral metabolism.
  • This profile does not measure calcium, phosphate, parathyroid hormone or kidney function.
  • Monitoring plans should follow the underlying condition and prescribed treatment.
  • Dietitian or specialist input may be needed when malabsorption is established.
People with limited Vitamin D sources
  • Limited outdoor exposure can reduce the skin’s opportunity to produce Vitamin D3.
  • Covering most skin, season and latitude can also reduce ultraviolet exposure.
  • Darker skin pigmentation can reduce Vitamin D production from a given exposure.
  • Older skin generally produces less Vitamin D3 than younger skin.
  • Few foods naturally contain substantial Vitamin D without fortification.
  • These factors indicate risk but do not prove a low blood concentration.
  • Sun exposure advice must also consider skin-cancer prevention and individual circumstances.
  • Testing decisions should follow clinical need rather than risk factors alone.
People taking D2 or D3 treatment
  • Separate fractions can help show whether D2 or D3 contributes to treatment response.
  • Bring the supplement or prescription label when the product form is uncertain.
  • Record the dose, schedule, start date and timing of the last dose.
  • Adherence, absorption and treatment duration can all affect the measured response.
  • A total that remains low does not prove that the prescribed product failed.
  • The clinician may review dose accuracy, adherence, absorption and related medical conditions.
  • Repeat timing should allow enough time for the planned treatment response.
  • Do not switch between D2 and D3 products solely from one fraction result.
People at risk from excessive supplementation
  • Very high supplement intake can raise total Vitamin D and blood calcium.
  • Vitamin D toxicity usually relates to excessive supplements rather than ordinary sunlight exposure.
  • Possible effects include nausea, weakness, thirst, frequent urination and kidney problems.
  • Symptoms are non-specific and require calcium and kidney assessment when concerning.
  • This profile does not include serum calcium or kidney-function measurements.
  • High-dose therapy needs a defined indication, dose plan and monitoring schedule.
  • Do not combine multiple Vitamin D products without checking their total dose.
  • Confusion, repeated vomiting or marked dehydration needs prompt clinical assessment.

Preparation And Interpretation Notes

Follow the profile collection instructions
  • Fasting is not required for this three-result Vitamin D Profile.
  • Follow stricter instructions when another booked test requires fasting.
  • A trained professional collects venous blood and prepares serum for analysis.
  • Maintain ordinary food and fluid habits unless a clinician advises otherwise.
  • Do not take an extra supplement dose to improve the test result.
  • Minor discomfort or bruising can follow a routine venous blood draw.
  • Tell the collector about fainting, difficult draws or blood-thinning medicines.
  • Preparation for another Vitamin D method may differ from this profile.
Document supplements and medicines
  • List every Vitamin D, calcium and multivitamin product before interpretation.
  • Identify whether each product contains D2, D3 or an unspecified form.
  • Record the strength in international units or micrograms and the dosing schedule.
  • Mention prescription loading doses, injections or combination bone-health products.
  • Some medicines can alter Vitamin D metabolism or absorption over time.
  • Report anticonvulsants, glucocorticoids and medicines used for fat-malabsorption when relevant.
  • Do not stop prescribed treatment unless the treating clinician advises it.
  • The last-dose timing helps compare results across repeated measurements.
Share diet, sunlight and health context
  • Describe major changes in outdoor exposure, diet or fortified-food intake.
  • Mention recent prolonged illness, reduced mobility or major weight change.
  • Share known liver, kidney, digestive, parathyroid or bone conditions.
  • Report pregnancy, older age or previous bariatric surgery when relevant.
  • Seasonal variation may affect D3 contribution and the total result.
  • These factors provide context and do not automatically invalidate the sample.
  • A recent short sunny period does not guarantee an adequate total concentration.
  • Avoid unsafe ultraviolet exposure solely to change a laboratory number.
Interpret total, fractions and units
  • Review the total result first because it represents combined D2 and D3.
  • Then compare each fraction with the reported supplement form and clinical history.
  • A low D2 value can coexist with an adequate D3-driven total.
  • A detectable D2 value may reflect ergocalciferol intake rather than a disorder.
  • Check whether the report uses nanograms per millilitre or nanomoles per litre.
  • Do not compare numbers across different units without a correct conversion.
  • Interpretive thresholds vary among laboratories and professional guidance.
  • Use trends cautiously when the laboratory method or supplement regimen changes.
Know the profile limits and next steps
  • This profile does not include active 1,25-dihydroxyvitamin D.
  • Active Vitamin D testing answers different kidney and calcium questions.
  • Calcium, phosphate, alkaline phosphatase and parathyroid hormone are not included.
  • Kidney and liver tests may be relevant when conversion or toxicity is questioned.
  • A clinician may repeat testing or add targeted investigations before changing treatment.
  • Do not self-start a high-dose course from one low result.
  • Do not continue excessive doses when a high result or toxicity is suspected.
  • Severe vomiting, confusion or dehydration needs prompt medical assessment.

Frequently Asked Questions

What does Vitamin D Profile include?

It includes Vitamin D2, Vitamin D3 and their combined total.

Is fasting required for this profile?

No; fasting is unnecessary unless another booked test requires it.

Which result shows overall Vitamin D status?

The combined total is usually the main status result.

Why are D2 and D3 reported separately?

They help show the contributions from different sources and supplements.

Does this include active Vitamin D?

No; 1,25-dihydroxyvitamin D is not included.

Does everyone need routine Vitamin D testing?

No; testing is most useful when a clinical indication exists.

Can supplements affect the result?

Yes; form, dose, schedule and treatment duration affect the pattern.

Does this profile include calcium?

No; calcium and related mineral tests are separate.

Can I book home collection?

Home collection is available where the entered address is serviceable.

Who should review my report?

A clinician should interpret the total with treatment and health history.

When should Vitamin D testing be repeated?

Repeat timing depends on the indication, treatment dose and previous result.

Sources: Vitamin D Test, 25-Hydroxy Vitamin D Test, Vitamin D Health Professional Fact Sheet, 25-Hydroxyvitamin D2 and D3, Serum, Vitamin D for the Prevention of Disease Guideline, Vitamin D Fact Sheet for Consumers

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Rs. 1910
Home Sample Collection Available No Extra Charges
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Why Choose Thyrocare for Vitamin D Profile

Get tested for Vitamin D Profile at fully automated diagnostic laboratory Thyrocare which has Centralized Processing Lab (CPL)
for esoteric tests and Regional Processing Labs in major cities of India.

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specimens and over 3 lakh Clinical investigations per day is the right choice for your Vitamin D Profile testing.

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Service for Vitamin D Profile test is available in all the major cities across India including Delhi, Pune, Kolkata, Chennai, Ghaziabad, Noida, Bangalore, Mumbai, Hyderabad, Gurgaon, Ludhiana, Cuttack, Coimbatore, Lucknow, Chandigarh, Bhopal, Nagpur, Gwalior, Amritsar, Indore, Jodhpur, Allahabad, Madurai, Guwahati, Ahmedabad, and 200 more tier 2 cities.

A Vitamin D profile test contains three main parameters, including Vitamin D total, Vitamin D2, and D3. Vitamin D is essential to maintain various functions of the body. It can be maintained with a balanced diet and required supplements. 

Vitamin D blood test measures the range of the abovementioned 3 vitamins in the body. It helps you know and understand your overall health and take appropriate measures on time. This test helps in monitoring the deficiency and high levels of vitamin D in the body. If you are experiencing any deficiency symptoms such as fatigue, weakness, bone and muscle weakness, loss of appetite, and cramps, it is time for you to get a Vitamin D test near you .

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